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| Ocarino,N.M.; Bozzi,A.; Pereira,R.D.O.; Breyner,N.M.; Silva,V.L.; Castanheira,P.; Goes,A.M.; Serakides,R.. |
| 4&', 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long extensions that apparently linked them to neighboring cells, while DAPI-treated cells were mostly round cells with fine and short extensions.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bone marrow; Cell cultures; Rat; Osteogenic differentiation. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200003 |
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